Safe and effective dose of ozone therapy in the treatment of abortions

An experiment was conducted at the Department of Obstetrics and Gynecology, Medical Academy, Nizhny, Novgorod and Central Research Laboratory, Medical Academy, Nizhny, Novgorod, Russia and Russian Association of Ozone Therapy Jordan to study the safe and effective dose of ozone therapy in the treatment of abortions.

In this experiment, blood from 45 women was drawn, these women were belonging to 3 distinct groups

1. 15 subjects were having threatened abortion.
2. 15 subjects were healthy, non pregnant women.
3. 15 subjects were pregnant without any abnormality.

20ml blood was drawn from each women and was further divided in 4 equal parts.

1. One part was left intact to be used as control.
2. Other 3 were treated with 0.4ml ozonated saline solution with concentrations of 400mcg/l, 800mcg/l,1200Mcg/l respectively.

Results:

The criteria for safety and efficiency of ozone therapy was considered to be intensification of antioxidant defense mechanism,that protects against free radical damage, and a decrease in products of free radical injury (lipid peroxidation) measured by presence of certain products of lipid per oxidation known as Conjugated dienes.

• Products of free radical injury which were measured to be 2.78nmol/mg in intact untreated blood showed a slight improvement at 400mcg/l and 800mcg/l and became 2.23mcg/l and 2.22mcg/l respectively but at concentration of 1200mcg/l it showed a significant worsening of condition and became 3.05nmol/l showing significant free radical injury.
• Products of free radical injury in patients with threatened abortion which were 6.72nmol/mg in intact untreated blood improved and became 5.51nmol/mg at 400mcg/l but keep on worsening at higher concentrations of 800mcg/l and 1200mcg/l and became 9.81nmol/mg and 11.22nmol/mg respectively.

Similarly antioxidant activity showed following trend:

• Antioxidant activity in healthy pregnant women which was 0.15 rel.U in intact untreated blood showed improvement and became 1.64 rel.U and 0.16rel.U at 400mcg/l and 800mcg/l respectivly. The levels of antioxidant activity at high concentrations ozone solutions did not show any significant change and became 0.154 rel.U.
• Antioxidant activity in healthy non pregnant women which was 0.12 rel.U in intact untreated blood showed improvement and became 1.74 rel.U and 0.18rel.U at 400mcg/l and 800mcg/l respectively. The levels of antioxidant activity at high concentrations ozone solutions did not show any significant change and became 0.12 rel.U.
• Antioxidant activity in patients with threatened abortion which was 0.065 rel.U in intact untreated blood showed significant improvement and became 1.74 rel.U while no significant change was seen at 800mcg/l and 1200mcg/l.

Conclusions:

These experiments proved applicability of ozone therapy in treatment of various complications of pregnancy and fetal diseases but within a narrow range of 400mcg/l and 800mcg/l concentrations.

Efficiency of topical ozone treatment in patients of old diabetes with diabetic ulcers of lower limbs.

A study was held at the Division of Plastic and reconstructive Surgery, Banai-Zon Medical Center, Haifa to assess the efficiency of topical ozone treatment along with other conventionally used treatment modalities in patients of old diabetes with diabetic ulcers of lower limbs.

For the sake of this study, 183 diabetic patients with lower limb diabetic ulcers were given topical ozone treatment along with their normal conventional treatments and were followed over a period of 4 years to see if there would be any benefit in adding ozone therapy to the conventional treatments.

Treatment that patients received:

• Topical and systemic antibiotics.
• Periodic wound cleaning.
• Changing dressings when needed.
• Topical Ozone application. 3 x per week
• Taking wound cultures for detection of bacterial infections.

Results:

• Prior to the usage of topical ozone as treatment, the average healing time of the wound was 14.1 months which after the treatment became only 49 days or 1.5 months.
• Out of all the patients that were followed, 84.8% were fully healed.
• Remaining patients were either discontinued from the trials;skins grafted or were diagnosed as non-responsive patients.
• A very small number of patients (4.3%) had to undergo limb amputation.

This study concluded that when topical ozone is used along with other modalities of treatment for diabetic ulcers of lower limbs is very beneficial.

This study concluded that when ozone was added to the normal conventional protocol, the wounds healed 9.5 times faster then without ozone topical treatment. This is the measurable what is the most important part of all

Efficiency of Ozone therapy as treatment of patients with Diabetic foot.

A study conducted at The University of Havana, Cuba and Ozone research center, Cuba. In collaboration with University of Milan, Italy and Institute of Angiology and Vascular Surgery, Cuba to prove the efficiency of Ozone as treatment of patients with Diabetic foot.

In this study a total of 100 diabetic patients were selected which were all having ulcers of feet and toes. The patients were divided into 2 groups:

1. A group of 49 patients which were treated with antibiotics only.
2. Another group of 51 patients which were treated with Ozone, administered via rectal and topical routes in 20 different sessions.

Parameters considered were:

• Appearance of wound before and after the treatment in both study groups
• Blood glucose levels at the start and end of the study.
• Antioxidant and enzyme levels before and after therapy.

Results:

• The average blood glucose levels in patients of both groups before study were 10.11(+-4.2)mM.
• After giving treatment allocated to both the groups, improvement in average blood glucose levels was seen in 84% patients belonging to the group that received ozone treatment, and only 40% patients of the group receiving antibiotics.
• After the treatment,the blood glucose levels of ozone treated patients were within the normal range I.e. less than 7.8mM while the Blood glucose levels of patients given antibiotics were still higher than the normal range.
• The wounds of the patients that received ozone treatment were healed two times faster as compared to the wounds of the patients which were given antibiotics.
• The duration of hospitalization was also significantly reduced in the patients of ozone group as compared to antibiotic group.
• Oxidant levels, which were approximately 142µM before the treatment were brought back to normal i.e. 103.7(+-17.7)µM in Ozone group, while no decrease in oxidant levels were seen in antibiotic group.
• Other enzyme parameters were also improved significantly in ozone treated patients as compared to patients treated with antibiotics

Conclusion:

The study proved that diabetic patients with foot ulcers which were treated with ozone not only had 25% less expenditure on treatment but also showed a very significant and quick improvement of sign and symptom and biochemical parameters as compared to patients that received only antibiotics.