Safe and effective dose of ozone therapy in the treatment of abortions

An experiment was conducted at the Department of Obstetrics and Gynecology, Medical Academy, Nizhny, Novgorod and Central Research Laboratory, Medical Academy, Nizhny, Novgorod, Russia and Russian Association of Ozone Therapy Jordan to study the safe and effective dose of ozone therapy in the treatment of abortions.

In this experiment, blood from 45 women was drawn, these women were belonging to 3 distinct groups

1. 15 subjects were having threatened abortion.
2. 15 subjects were healthy, non pregnant women.
3. 15 subjects were pregnant without any abnormality.

20ml blood was drawn from each women and was further divided in 4 equal parts.

1. One part was left intact to be used as control.
2. Other 3 were treated with 0.4ml ozonated saline solution with concentrations of 400mcg/l, 800mcg/l,1200Mcg/l respectively.

Results:

The criteria for safety and efficiency of ozone therapy was considered to be intensification of antioxidant defense mechanism,that protects against free radical damage, and a decrease in products of free radical injury (lipid peroxidation) measured by presence of certain products of lipid per oxidation known as Conjugated dienes.

• Products of free radical injury which were measured to be 2.78nmol/mg in intact untreated blood showed a slight improvement at 400mcg/l and 800mcg/l and became 2.23mcg/l and 2.22mcg/l respectively but at concentration of 1200mcg/l it showed a significant worsening of condition and became 3.05nmol/l showing significant free radical injury.
• Products of free radical injury in patients with threatened abortion which were 6.72nmol/mg in intact untreated blood improved and became 5.51nmol/mg at 400mcg/l but keep on worsening at higher concentrations of 800mcg/l and 1200mcg/l and became 9.81nmol/mg and 11.22nmol/mg respectively.

Similarly antioxidant activity showed following trend:

• Antioxidant activity in healthy pregnant women which was 0.15 rel.U in intact untreated blood showed improvement and became 1.64 rel.U and 0.16rel.U at 400mcg/l and 800mcg/l respectivly. The levels of antioxidant activity at high concentrations ozone solutions did not show any significant change and became 0.154 rel.U.
• Antioxidant activity in healthy non pregnant women which was 0.12 rel.U in intact untreated blood showed improvement and became 1.74 rel.U and 0.18rel.U at 400mcg/l and 800mcg/l respectively. The levels of antioxidant activity at high concentrations ozone solutions did not show any significant change and became 0.12 rel.U.
• Antioxidant activity in patients with threatened abortion which was 0.065 rel.U in intact untreated blood showed significant improvement and became 1.74 rel.U while no significant change was seen at 800mcg/l and 1200mcg/l.

Conclusions:

These experiments proved applicability of ozone therapy in treatment of various complications of pregnancy and fetal diseases but within a narrow range of 400mcg/l and 800mcg/l concentrations.